METHODS: We performed a nested case control study within the population based prospective Golestan Cohort Study 50,045 participants, followed up to 14 years and assessed TERT promoter mutations in baseline urine samples from 38 asymptomatic individuals who subsequently developed primary BC and 152 matched controls using a Next Generation Sequencing based single plex assay UroMuTERT and droplet digital PCR assays. ...