Pakistan has the second highest burden of Hepatitis C in the world. A total of 683 individuals, who visited liver clinic during the study period, were screened for the presence of HCV infection. 534 individuals showed positive HCV infection were grouped in case group and 149 individuals with HCV negative status were grouped in control group. ...These results were further expanded by regression analysis that showed family history and sexual history are independent risk factors for transmission of hepatitis C infection and mother's history has no significance as risk factor for transmission. ...