There are no data on the farm level prevalence and risk factors of HEV in ruminant farms in Jordan or elsewhere. This study aimed to estimate the seroprevalence and risk factors of HEV in ruminant farms in all regions of Jordan. ...CONCLUSIONS: This study shows widespread and high farm level HEV seroprevalence in dairy farms in Jordan. Husbandry practices and off abattoir carcass processing in Jordan could amplify emergence and transmission of zoonotic HEV. ...